Purification of Glutathione Reductase from Some Tissues of Capoeta umbla and The Inhibitory Effects of Some Metal Ions on Enzyme Activity

The aim of this study was to determine the in vitro inhibitory effects some metal ions (silver ion (Ag+), cadmium (Cd2+), cobalt (Co2+), copper (Cu2+), nickel (Ni2+), lead (Pb2+) and zinc (Zn2+)) on glutathione reductase (GR) enzyme activities that purified from gill, kidney liver tissues Capoeta umbla. For purpose, C. umbla freshwater fish using ammonium sulfate precipitation affinity column chromatography methods 2′,5′-ADP Sepharose 4B. Within study, GR for first time Enzyme purity molecular weight were determined sodium dodecyl polyacrylamide gel electrophoresis method. In addition, different (Ag+, Cd2+, Co2+, Cu2+, Ni2+, Pb2+ Zn2+) tissue investigated under conditions. concentrations inhibiting 50% activity (IC50) obtained by plotting percentage versus [I] figures. Finally, dissociation constants inhibitor complex (Ki), inhibition types, calculated Lineweaver–Burk plots. rank order as Ag+>Co2+>Pb2+>Zn2+>Cu2+ gill GR; Ag+>Pb2+>Co2+> Ni2+>Zn2+ Ag+>Cu2+>Co2+>Pb2+>Ni2+ GR. From these results, we showed Ag+ is most potent tissues. Our results also demonstrate metals might be dangerous at low micromolar enzyme.